The effectiveness of a gamification-based student worksheet in improving students’ learning outcomes on the human immune system

Karyn Anastasia Siagian; Aida Fitriani Sitompul

doi:10.21831/edubio.v13i1.30276

The effectiveness of a gamification-based student worksheet in improving students’ learning outcomes on the human immune system

Karyn Anastasia Siagian, Universitas Negeri Medan, Indonesia
Aida Fitriani Sitompul, Universitas Negeri Medan, Indonesia

10.21831/edubio.v13i1.30276

Abstract

This study aimed to determine the effectiveness of a gamification-based student worksheet in improving students’ learning outcomes on the human immune system. A quantitative approach with a one-group pretest–posttest design was employed, involving 36 students from Class XI-12 at SMA Negeri 1 Medan. The intervention was conducted over three learning sessions. Students’ learning outcomes were measured using a 20-item multiple-choice test selected through expert validation and empirical item analysis. The data were analyzed using the normalized gain (N-Gain). The results showed that the mean test score increased from 56.52 on the pretest to 88.47 on the posttest, representing an increase of 31.95 points. The mean N-Gain was 0.7615 (76.15%), which was classified as high based on the criteria used in this study. These findings indicate that the gamification-based student worksheet was effective in improving students’ learning outcomes on the human immune system within the context of this study. The worksheet integrated communicative explanations, supporting illustrations, step-by-step descriptions of immune mechanisms, and gamification elements, including levels, challenges, and rewards. The developed worksheet can serve as an alternative instructional resource for Biology teachers teaching the human immune system and as a reference for developing similar worksheets for other Biology topics, with appropriate adaptations to learning objectives and topic characteristics. Future research should involve larger and more diverse samples and appropriate comparison groups to strengthen the evidence of effectiveness. Further studies may also investigate students’ learning motivation, engagement, and conceptual understanding, as well as assess learning retention through delayed posttests, to provide a more comprehensive understanding of the potential benefits of gamification-based student worksheets in Biology learning.

Keywords

Biology learning; Gamification-Based student worksheet; Human immune system; Learning outcomes

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References

Aini, F., & Husna, N. (2025). Penggunaan Elemen Gamifikasi dalam Meningkatkan Motivasi dan Keterlibatan Siswa dalam Pembelajaran. Sosial Khatulistiwa: Jurnal Pendidikan IPS, 05(01), 63–76. https://jurnal.untan.ac.id/index.php/JPIPS/article/view/93110/0

Arafah, N., & Sudrajat, A. K. (2026). Analysis of learning difficulties of students in regular and sports-specific classes (SSC) on cell material. Jurnal Edukasi Biologi, 12(1), 10–20. https://doi.org/10.21831/edubio.v12i1.25235

Batubara, A. J. A., Matanari, N. D., & Simanjuntak, M. P. (2025). Analisis Kebutuhan dan Pengembangan LKPD Berbasis Problem Based Learning (PBL) dalam Pembelajaran IPA di SMP Negeri 2 Percut Sei Tuan. Invention: Journal Research and Education Studies, 6(3), 950–960. https://doi.org/10.51178/invention.v6i3.2918

Chans, G. M., & Castro, M. P. (2021). Gamification as a Strategy to Increase Motivation and Engagement in Higher Education Chemistry Students. Computers, 10(132), 1–25. https://doi.org/10.3390/computers10100132

Chon, V. B. F. A., Sharif, S. bte, & Saleh, N. (2024). Gamification for Enhancing Students’ Learning Motivation : A Systematic Review. Journal of Cognitive Sciences and Human Development, 10(2), 19–41. https://doi.org/10.33736/jcshd.7231.2024

Christopoulos, A., & Mystakidis, S. (2023). Gamification in Education. Encyclopedia, 3(1), 1223–1243. https://doi.org/10.3390/encyclopedia3040089

Inanna, I., Rahmatullah, R., & Hasan, M. (2021). Evaluasi Pembelajaran: Teori Dan Praktek. Makassar. Tahta Media Group.

Jannah, G. R., & Sudrajat, A. K. (2026). The effect of project-based learning (PjBL) assisted by ChatGPT on critical thinking skills in climate change material. Jurnal Edukasi Biologi, 12(2), 213–223. https://doi.org/10.21831/edubio.v12i2.30113

Karima, A. I., Rahmawati, T., & Siswaningsih, W. (2026). Development of Reading and Numeracy Literacy Assessment Instrument in Electrolyte and Non-Electrolyte Solutions for Critical Thinking Skills. Jurnal Pendidikan MIPA, 16(4), 1633–1646. https://doi.org/10.37630/jpm.v16i4.4958

Kaune, N., Panigoro, H. S., & Majid. (2025). Analisis butir soal matematika materi persamaan linier dua variabel berbasis Google Sites di SMP Negeri 3 Telaga. Jurnal Pendidikan Matematika RAFA, 11(2), 131–145. https://garuda.kemdiktisaintek.go.id/documents/detail/6003613

Lewar, A. K., Sada, M., & Bulan, S. (2025). Pengembangan Video Pembelajaran Materi Sistem Imun untuk Siswa Kelas XI SMA. Jurnal Inovasi Pembelajaran Biologi, 6(1), 41–51. https://doi.org/10.26740/jipb.v6n1.p42-52

Listiani, N. P. A., Soeprianto, H., Salsabila, N. H., & Subarinah, S. (2024). Efektivitas Media Pembelajaran Interaktif Berbasis Articulate Storyline 3 terhadap Hasil Belajar Matematika Siswa Kelas XI SMA. Jurnal Pendidikan MIPA, 14(3), 682–692. https://doi.org/10.37630/jpm.v14i3.1761

Magdalena, I., Fauziah, S. N., Faziah, S. N., & Nupus, F. S. (2021). Analisis Validitas, Reliabilitas, Tingkat Kesulitan dan Daya Beda Butir Soal Ujian Akhir Semester Tema 7 Kelas III SDN Karet 1 Sepatan. BINTANG: Jurnal Pendidikan Dan Sains, 3(2), 198–214. https://ejournal.ipn.ac.id/index.php/bintang/article/view/1291

Munawir, Rofiqoh, A., & Khairani, I. (2024). Peran Media Interaktif Dalam Meningkatkan Motivasi Belajar Siswa pada Mata Pelajaran SKI di Madrasah Ibtidaiyah. Jurnal AL-Azhar Indonesia Seri Humaniora, 9(1), 63–71. http://dx.doi.org/10.36722/sh.v9i1.2828

Nestiadi, A., Safitri, A., Aulia, F., Maulidya, R. R., & Hasanah, N. (2024). Penggunaan LKPD untuk Menunjang Keaktifan Peserta Didik Pada Pembelajaran IPA di SMPN 1 Ciruas. Gudang Jurnal Multidisiplin Ilmu, 2(10), 337–340. https://doi.org/10.59435/gjmi.v2i11.1091

Purnamasari, A. (2020). Sistem Pertahanan Tubuh Biologi-Kelas XI. Direktorat SMA, Direktorat Jenderal PAUD, DIKDAS dan DIKMEN.

Rahman, M. H. A., Panessai, I. Y., Noor, A. Z. M., & Salleh, N. S. M. (2018). Gamification Elements and Their Impacts on Teaching and Learning - A Review. The International Journal of Multimedia & Its Applications (IJMA), 10(6), 37–46. https://aircconline.com/abstract/ijma/v10n6/10618ijma04.html

Ramli, R. A., & Nurhidayah. (2024). Penerapan Strategi Gamifikasi Dalam Mengoptimalkan Hasil Belajar Siswa Kelas XI MIPA 6 SMAN 4 Maros. Jurnal Binomial Pendidikan Biologi, 7(2), 42–51. https://ejournals.umma.ac.id/index.php/binomial/article/view/2414

Ratinho, E., & Martins, C. (2023). The role of gamified learning strategies in student’ s motivation in high school and higher education : A systematic review. Heliyon, 9(1), 1–16. https://doi.org/10.1016/j.heliyon.2023.e19033

Sailer, M., & Homner, L. (2020). The Gamification of Learning : a Meta-analysis. Educational Psychology Review, 32(1), 77–112. https://doi.org/10.1007/s10648-019-09498-w

Sari, I. P. N., & Ferry, D. (2024). Analisis Kesulitan Belajar Siswa pada Mata Pelajaran Biologi di SMA. Biosfer: Jurnal Biologi Dan Pendidikan Biologi, 9(2), 172–181. https://doi.org/10.23969/biosfer.v9i2.18613

Selçuk, Z. V., & Keskin, M. Ö. K. (2024). Gamification in biology education: a systematic review analysis. International Journal of Eurasia Social Sciences, 15(55), 375–405. http://dx.doi.org/10.35826/ijoess.4445

Shabrina, A., Putri, R., & Khairi, A. (2025). Pentingnya Pemilihan Media Pembelajaran Yang Tepat Untuk Meningkatkan Hasil Belajar Siswa. Zaheen : Jurnal Pendidikan, Agama Dan Budaya, 1(2), 120–131. https://jurnalinspirasimodern.com/index.php/Zaheen/article/view/108

Sugiyono. (2023). Metode Penelitian Kuantitatif, Kualitatif, dan R&D. Alfabeta Bandung.

Syafitriyani, D., Komariah, & Wahyuningsih, Y. (2023). Rancang Bangun Media Pembelajaran Interaktif Articulate Storyline Mata Pelajaran IPS Jenis-Jenis Pekerjaan Sekolah Dasar. Pendas: Primary Edication Journal, 4(2), 105–118. https://doi.org/10.29303/pendas.v4i2.1509

Syarah, M. M., Rahmi, Y. L., & Darussyamsu, R. (2021). Analisis Penerapan Pendekatan STEM Pada Pembelajaran Biologi. BIO-EDU: Jurnal Pendidikan Biologi, 6(3), 236–243. https://doi.org/10.32938/jbe.v6i3.1260

Taber, K. S. (2018). The use of Cronbach’s alpha when developing and reporting research instruments in science education. Research in Science Education, 48(6), 1273–1296. https://doi.org/10.1007/s11165-016-9602-2

Widodo, S., Ladyani, F., Asrianto, L. O., Rusdi, R., Khairunnisa, K., Lestari, S. M. P., Devriany, A., Wijayanti, D. R., Hidayat, A., Dalfian, D., Nurcahyati, S., Sjahriani, T., Armi, A., Widya, N., & Rogayah, R. (2023). Buku Ajar Metode Penelitian. CV Science Techno Direct.

Yadnyawati, I. A. G. (2019). Evaluasi Pembelajaran. UNHI Press.

Yuningsih, & Susilo, M. J. (2018). Kajian Pendekatan Analogi dalam Pembelajaran Biologi yang Bermakna. BRILIANT: Jurnal Riset Dan Konseptual, 3(3), 268–279. https://doi.org/10.28926/briliant.v3i3.188

DOI: https://doi.org/10.21831/edubio.v13i1.30276