KOMBINASI NANOPARTIKEL PERAK IONIK DAN EKSTRAK DAUN KELOR SEBAGAI BAHAN ANTIBAKTERI UNTUK Pseudomonas aeruginosa

Ambarwati Purwaningrum, Universitas Negeri Yogyakarta, Indonesia, Indonesia

 10.21831/fisika - s1.v10i2.20167

Abstract


This article reports on research aimed at determining the influence of the concentration of ionic silver nanoparticles, the concentration of flavonoid extract from moringa leaves, and their combination on the inhibitory effect and duration of inhibition of Pseudomonas aeruginosa bacterial growth. Ionic silver nanoparticles (AgNp) were synthesized using the electrolysis method, and moringa leaf extract (Moringa Oleifera) was obtained using the decoction method. Characterization of ionic silver nanoparticles was carried out using UV-Vis Spectrophotometer, AAS, and PSA, while the characteristics of moringa leaf extract were analyzed using Visible Spectrophotometer and PSA. The test samples used were AgNp 50ppm, Flavonoid 50ppm, AgNp 10ppm + Flavonoid 40ppm, AgNp 20ppm + Flavonoid 30ppm, AgNp 25ppm + Flavonoid 25ppm, AgNp 30ppm + Flavonoid 20ppm, and AgNp 40ppm + Flavonoid 10ppm, which were subsequently tested on Pseudomonas aeruginosa bacteria using the Kirby Bauer method. The results of the inhibitory effect and duration of inhibition showed that the higher the concentration of ionic silver nanoparticles and the lower the concentration of flavonoid in the combination, the larger the diameter formed, and the longer the inhibitory effect on Pseudomonas aeruginosa bacteria. Ionic silver nanoparticles with a concentration of 50ppm formed a diameter of 10.21mm for more than 72 hours, and moringa leaf extract with a flavonoid concentration of 50ppm formed a diameter of 7.49mm with a duration of inhibition of up to 51 hours. Meanwhile, the best results were obtained in the sample combination of AgNp 40ppm + Flavonoid 10ppm, which formed a diameter of 11.44mm with an inhibitory effect lasting more than 72 hours. However, the best inhibitory effect results were still below the capability of chloramphenicol as a positive control, which formed an inhibitory zone with a diameter of 14.16 mm that could be sustained for 72 hours.

Keywords


Ionic silver nanoparticles, moringa leaf extract (Moringa Oleifera), electrolysis, flavonoid, Kirby Bauer, Pseudomonas aeruginosa

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DOI: https://doi.org/10.21831/fisika%20-%20s1.v10i2.20167

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